You'd be proud of me.

I realized why I didn't get any colonies from my transformation, and it's because I made a stupid mistake and now I'm set back a day and I'm not going to be able to leave lab until after midnight tonight.
And it was a silly mistake. I did steps A through D but then accidentally used the stuff from step C to do step E.

But I didn't beat myself up over it like I normally would. I mean, I wrote "So I'm and idiot and [explanation of what the mistake was], ugh" in my lab notebook, but it was in an annoyed way, not a self loathing way.
Honestly I'm a bit relieved to have an explanation. If it still doesn't work and I can't figure out why that means troubleshooting with a million variables.

Still not going to say nice things to myself though... Maybe if I actually have transformants tomorrow.